Phosphomotif antibodies
WebProtocols. As the epigenetics experts, Active Motif continues to develop new methods and helpful techniques to support your research. Our goal is to ensure your success with everything from gold standard assays to emerging approaches for epigenetics analysis. Check back often as we add new protocols, technotes and guides to this resource. WebApr 13, 2024 · Figure 1.Expression levels of obscurin in wild-type and Obscn-ΔIg58/59 atria.(A,A’) Immunoblotting (A) and relative quantification (A’) of giant obscurin in wild-type 12-month-old left ventricles (LV) and atrial lysates utilizing antibodies to Ig67 did not reveal significant expression differences; t-test, p = 0.18.(B) Immunoblotting of giant obscurin in …
Phosphomotif antibodies
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WebApr 1, 2009 · Phosphomotif antibody 5557 is phosphospecific and detects MAPK phosphorylation events.A, sequence of phosphopeptide library containing two consecutive consensus MAPK phosphorylation sites used for immunization to produce antibody 5557. Webphosphomotif antibodies, indicating the pos-sibility of direct ERK substrates. From this screen, 38 candidate targets were identi-fied by mass spectrometry (MS), includ-ing 13 new substrates that were verified by in vitro ERK phosphorylation. Further stud-ies of one of them, the nucleoporin Nup50, revealed an unexpected role of ERK signal-
WebThe antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns. Storage: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and … Webby a recent study in which phospho-motif specific antibodies were used to enrich for peptides containing pS/pT-Q, with the goal of identifying substrates of SQ/TQ-directed kinases (e.g., ... including cell line and phosphospecific-versus-phosphomotif antibody differences. The phosphorylation sites identified in both data sets are of
WebDownload scientific diagram Phosphomotif antibody 5557 is phosphospecific and detects MAPK phosphorylation events. A, sequence of phosphopeptide library containing two … WebMay 13, 2015 · The ability of anti-mHttexon1 antibodies and antibody fragments to reduce the aggregation and associated neurotoxicity has proven to be strongly dependent on the targeted epitope . Specifically targeting the Htt sequence between 1 and 17, which has been shown to play a key role in aggregation and HD pathology, seems to be an obvious choice.
WebNov 11, 2013 · Here we have used phosphomotif antibodies and mass spectrometry (MS) to investigate the effects of MEK/PI3K dual inhibition during the period immediately preceding cell death. Upon treatment, melanoma cell lines responded by dramatically increasing phosphorylation on proteins containing a canonical DNA damage-response (DDR) motif, …
WebAntibodies—A key reagent for this study is the antibody for the Akt phosphomotif RXRXXpS/T, where X is any amino acid. This antibody, which is referred as the PAS antibody (phospho Akt substrate), was purchased from Cell Signaling Technology, Beverly, MA (catalog num-ber 9611). It is an affinity-purified rabbit antibody that has been shown rick dennehy obituaryWebnonphosphorylatable Ser172Ala mutation abolished interaction with the antibody to the 14-3-3 binding motif, whereas a nonphosphorylatable Ser155Ala mutation abolished the interaction with the antibody to the MPK substrate motif, and mutation of either site alone did not affect the AICAR-induced phosphorylation of the other site (Fig. 3C). rick demchuk obituaryWebPhosphoNET is an open-access, online resource developed by Kinexus Bioinformatics Corporation to foster the study of cell signalling systems to advance biomedical research in academia and industry. PhosphoNET is the worlds largest repository of known and predicted information on human phosphorylation sites, their evolutionary conservation, the ... red shirt glasses memeWebAntiphospholipid antibodies are antibodies directed against phosphorus-fat components of your cell membranes called phospholipids, certain blood proteins that bind with … rick denton facebookWebPhosphosite-specific antibodies can be produced as polyclonals or monoclonals in different animal species, and each approach offers its own benefits and disadvantages. The … rickderringer.comWebNov 26, 2013 · Here we have used phosphomotif antibodies and mass spectrometry (MS) to investigate the effects of MEK/PI3K dual inhibition during the period immediately preceding cell death. rick dejesus wifeWebantibodies, generally crosslinked to a resin, targeting a consensus phosphomotif or a specific phosphoresidue, such as phosphotyrosine (pTyr) 13,14. This last option is highly specific but is generally limited due to the cost of use. IAP can be less efficient in complex samples due to the high background red shirt friday tank top