Hygromycin phosphotransferase hpt gene

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http://www.dictall.com/indu/081/0804045A1C1.htm Webfragment containing alox site, a promoterless hpt (hygromycin phosphotransferase coding region) gene and a nos 3 ′ terminator from plasmid p24 was isolated, gel purified and ligated on the

hygromycin B 4-O-kinase 2.7.1.163 Request PDF

WebFatty acid (FA) composition determines the quality of oil from oilseed crops, and thus is a major target for genetic improvement. FAD2 (Fatty acid dehydrogenase 2) and FAE1 (fatty acid elongase 1) are critical FA synthetic genes, and have been the focus of genetic manipulation to alter fatty acid composition in oilseed plants. In this study, to improve the … WebA total of 3.46 x 10(8) protoplasts isolated from 17 independent transgenic Arabidopsis lines harbouring the defective chimeric hpt gene were transformed via direct gene … byruby fish pie

Efficient transformation of Arabidopsis thaliana using direct gene ...

WebSelectable reporter gene Antibiotic Resistance Genes Neomycin phosphotransferase II (npt II gene) • Derived from the transposon Tn5 code foraminoglycoside 3` … Web列表数据仅在虚线下方。全文数据即将推出。 WebRui Zhang is an academic researcher. The author has contributed to research in topic(s): Cotyledon & Transformation (genetics). The author has an hindex of 1, co-authored 1 publication(s) receiving 10 citation(s). by rubbing

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WebThe aim of this work is to evaluate the use of the hygromycin phosphotransferase (hpt) gene as the selectable marker for generation of stable oil palm transformation via … WebObjective This article studied on the food safety of HPT protein (Hygromycin B Phosphotransferase), a kind of plant selectable marker gene expression product, which … clothing policy hln newsWebImmunogen: ABI30072.1 Synonyms: HPT Background: The hygromycin phosphotransferase (denoted hpt, hph or aphIV) gene was originally derived from … byrudor.com

"WebHPT / Anti-HPT Antibody. Starting at $89.00. Add to Wish List. Immunogen: ABI30072.1. Synonyms: HPT. Background: The hygromycin phosphotransferase (denoted hpt, … " - Hygromycin phosphotransferase hpt gene

Hygromycin phosphotransferase hpt gene

(Open Access) Transformation of the dermatophyte Trichophyton ...

Web1 dec. 1991 · Abstract Abstract A general method of gene transfer that does not leave behind a selectable marker in the host genome is described. A luciferase gene was … Web1. Digest the pCAMBIA 1301 by SphI (Fig. 5 D) and amplify the hygromycin resistance gene hptII (2146 bp, together with the promoter pCaMV35S, shown in Fig. 5 E) used as …

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Web5 jun. 2008 · Hundreds of hygromycin-resistant plants per sporangium were obtained by co-cultivation of immature thalli with Agrobacterium carrying the binary vector that … Web24 sep. 2011 · Of three major selectable genes, the bialaphos resistance (bar) gene and the hygromycin phosphotransferase (hpt) allowed effective selection of transformed cells …

Web3 jul. 2024 · The pHSE401-2gR-PdbPDS recombinant plasmid containing the Cas9 expression cassette, two sgRNA cassettes, and the hygromycin phosphotransferase … WebThus, it directly affects the tea industry qualitatively and quantitatively. Solanum tuberosum class I chitinase gene (AF153195) is a plant pathogenesis-related gene. It was introduced into tea genome via Agrobacterium-mediated transformation with hygromycin phosphotransferase (hpt) gene conferring hygromycin resistance as plant selectable …

Webcultivated with Agrobacterium strain EHA101 containing a binary vector pTCL5, having hygromycin phosphotransferase (HPT) gene which confers resistance to hygromycin … WebTransformation and inheritance of a hygromycin phosphotransferase gene in maize plants. Embryogenic maize (Zea mays L.) callus cultures were transformed by …

WebThe objective of this study was to determine the efficacy of three commonly used Agrobacterium strains in genetic transformation of the microalga Chlamydomonas reinhardtii. Three different strains of Agrobacterium tumefaciens (EHA 101, EHA 105, LBA

WebEmbryogenic calli induced after 20 days of highly regenerating rice cultivar BRRI dhan29 and Agrobacterium strain GV 3101 was transformed with binary vector pCAMBIA 1303 … clothing policy in childcareWeb1 feb. 2009 · Conclusion HPT protein does not have any safety problems to human health. Key words: Hygromycin B phosphotransferase; Selectable marker; Acute toxicity; … clothing policy college staffWeb7 aug. 2007 · ABSTRACT: The hygromycin B phosphotransferase gene (hpt) has been widely used in the process of plant genetic engineering to produce plants that can … by ruby food deliveryWebGene expression analysis was done by hygromycin antibiotic resistant test of leaf samples.Analysis was carried out on 17 lines of Tl transgenic rice plants from Batutegi … clothing pollutionhttp://lipi.go.id/publikasi/pewarisan-gen-penanda-hpt-hygromycine-phosphotransferase-berdasarkan-analisis-pcr-dan-ekspresinya-pada-populasi-padi-transforman-mengoverekspresikan-gen-hd-zip-oshox-6/6262 by ruby nw10 7pqWeb25 sep. 2015 · To date, however, only the hygromycin phosphotransferase gene (hpt) has been available for use as a marker for Agrobacterium-mediated transformation in M. … byruby londonWebmarker-off. The hygromycin phosphotransferase gene (hpt) from E. coli which has been proved to be very effective marker for plant transformation was used in this study. The … clothing pollution facts