Cy5 filter on compound
WebEmission filter (nm) Channel Antibody labels Compatible fluorescent proteins; Blue–488 nm: 525/50: BL1: Alexa Fluor 488 FITC: eGFP Emerald eYFP: 590/40: BL2: PE PE-Alexa Fluor 610 PE-Texas Red: EYFP mCitrine Venus: 695/40: BL3: PE-Alexa Fluor 700 PE-Cy5.5 PerCP PerCP-Cy5.5 Qdot 705 TRI-COLOR : Green–532 nm: 575/36: GL1: Alexa Fluor … WebThe TECHSPEC® Cy5™ Filter Cube Set - Olympus contains a dichroic filter, an emission filter, and an excitation filter designed for the Cy5 spectrum. The emission filter has a …
Cy5 filter on compound
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Cy5.5 is a near-infrared (IR) fluorescence-emitting dye (excitation/emission maximum 678/694 nm). Cy7 is a near-IR fluor that is invisible to the naked eye (excitation/emission maximum 750/776 nm). It is used in in vivo imaging applications, as well as the Cy7.5 dye. See more Cyanines, also referred to as tetramethylindo(di)-carbocyanines are a synthetic dye family belonging to the polymethine group. Although the name derives etymologically from terms for shades of See more Cyanines were first synthesized over a century ago. They were originally used, and still are, to increase the sensitivity range of photographic emulsions, i.e., to increase the range of See more The word cyanin is from the English word "cyan", which conventionally means a shade of blue-green (close to "aqua") and is derived from the Greek κυάνεος/κυανοῦς kyaneos/kyanous which means a somewhat different color: "dark blue". See more Cyanines have been classified in many ways: • Streptocyanines or open chain cyanines: R2N … See more Because they yield brighter and more stable fluorescence, cyanines can advantageously replace conventional dyes such as See more WebNov 13, 2011 · In contrast to the deoxygenated condition, Cy5-COT and Cy5-NBA performed better than Cy5-Trolox. Figure 3: Photostability of Cy5 and Cy5-TSQ conjugates in the presence of oxygen and in living cells.
WebThe correction factor (CF) for Cy5 (Cyanine-5) is CF 260 = 0.02, CF 280 = 0.03, CF 482 = 0.009, CF 565 = 0.09. To request additional information and datasets for Cy5 (Cyanine-5), please [email protected]. Unless otherwise specified, correction factor (CF) values are typically measured in ddH 2 O or PBS. WebCy5 HYQ - The Cy5 HYQ filter combination for red-wavelength excitation is designed to provide high-energy excitation of the fluorophore Cy5, although the wide excitation range (yellow-orange to red) allows the set to be utilized with a variety of fluorochromes. Sharp filter transitions between reflection and transmission regions enable ...
WebCy5 Filter Set. This Iridian single band filter set consists of the 628-40 EX, 692-40 EM and 660 DM filters. It is optimised for the cyanine dye Cy5. … WebCy5 HYQ - The Cy5 HYQ filter combination is the only Nikon set designed for high-energy red-wavelength excitation, and although optimized for use with the fluorophore Cy5, the wide yellow-orange to red excitation range is suitable for applications employing a variety of fluorochromes. Fluorescence generated at far-red to near-infrared ...
WebThe excitation filter was at 485 nm to excite Cy3 except for panel (c) as noted below. Emission was monitored in two channels: the Cy3 channel at 535 nm and the Cy5 channel at 665 nm.
WebAll filter sets tested permitted direct visualization of Cy5 labeling, which was seen as deep red signal over a very dark background for both immunostained and FISH preparations. Medium-high-power objectives (X20-100) were most effective, lower magnification resulting in little visible signal. play dough bath soapWebSurprisingly, I saw that the specimen labeled with Cy5 only was seen using the Cy3 method (532nm laser and BPG1 or DGR1 GE filter that cuts a 560-580 nm band) and that it looked half as bright as ... primary ed shuWebThe correction factor (CF) for Cy5 (Cyanine-5) is CF260 = 0.02, CF280 = 0.03, CF482 = 0.009, CF565 = 0.09. Unless otherwise specified, correction factor (CF) values are typically measured in ddH 2 O or PBS. Select a compound from the list below to view correction factor (CF) data. Enter keywords into the search box to filter the list. play dough bead microwaveWebBulk fluorescence measurements of the TSQ-conjugated Cy5 fluorophores demonstrated that absorption and emission spectra of TSQ-fluorophore conjugates were largely indistinguishable from those of the parent Cy5 compound, aside from modest shifts in fluorescence quantum yield (Supplementary Fig. 2a,b).The quantum yields for Cy5-COT, … playdough beesWebJan 10, 2024 · Alexa Fluor® dyes are a big group of negatively charged and hydrophilic fluorescent dyes, frequently used in fluorescence microscopy. All the Alexa Fluor® dyes are sulfonated forms of different basic fluorescent substances like fluorescein, coumarin, cyanine or rhodamine (e.g. Alexa Fluor®546, Alexa Fluor®633).The respective laser … playdough benefit for toddlersWebApr 10, 2024 · In penetration experiment, Cy5-LNP/Cy5-cLNP/ Cy5-rIGF-1 was added. For block group, CAQK free peptide was added prior to adding LNP/cLNP. After incubation, cartilage explants were immersed in PBS for 24 hours and embedded in Tissue-Tek OCT compound. The 6-μm-thick cryosections were cut and imaged with a fluorescence … primary education agesWebFeb 20, 2012 · Product overview. Annexin V-PE-Cy5 Apoptosis Staining / Detection Kit (ab14159) is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily … primary education act