WebhCMEC/D3 cells (passage 27–39) were cultured in Endothelial Cell Basal Medium-2 (Lonza, Walkersville, MD, USA), supplemented with vascular endothelial growth factor, insulin-like growth factor 1, epidermal growth factor, fibroblast growth factors, hydrocortisone, ascorbate, GA-1000, heparin, and 2.5% fetal bovine serum (FBS) and grown on rat ... WebProduct Overview Lonza's Cryopreserved HUVECs are shipped as frozen primaries. They are guaranteed to express CD31/105 and survive in culture through 15* population doublings (*except HUVEC-XLTM guaranteed through 5 PD ). All cells test negative for mycoplasma, bacteria, yeast, and fungi.
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