Invsc1 yeast strain

http://www.maokangbio.com/productView.action?id=10242 Web[0033] In order to show the coding function of the RrFAE1 gene, the present invention clones the RrFAE1 gene between the BamHI and KpnI sites of pYES2/NT C of Invitrogen, and transforms the yeast strain InvSc1 to obtain high expression.

IJMS Free Full-Text Genome-Wide Identification of Cassava ...

Web1 feb. 2024 · The commercial diploid S. cerevisiae strain INVSc1 (MATa his3D1 leu2 trp1-289 ura3-52 MAT his3D1 leu2 trp1-289 ura3-52) (Invitrogen, Carlsbad, CA, USA) was used as the host strain in this study. The yeast strain was routinely propagated at 30 °C in yeast extract peptone dextrose (YPD) medium or yeast synthetic minimal (SM) medium. WebThe INVSc1 Yeast Strain is included with each vector kit. Note: For long-term storage of your stab, we recommend preparing a glycerol stock immediately upon receipt and … flocked nutcracker https://susannah-fisher.com

INVSC1 Yeast Strains - gen.store

Web25 apr. 2000 · The strains used in this study were INVSc1 (Invitrogen), DEY1453 , and smf1 (MATα his3 ade2 leu2 trp1 ura3smf1∷URA3ura3∷TRP1). This smf1 strain was derived from the smf1 from Supek et al. ( 22 ) by transformation with linearized pRH530, a plasmid containing ura3∷TRP1 , and selection for cells that were trp + and ura − . Web9 apr. 2024 · The growth activity of CqTPS4-and CqTPS8-expressing yeast under saline-alkali stress. Yeast strain INVSC1 was transformed with the pYES2-CqTPS4, pYES2-CqTPS8, or pYES2 empty vector (control), respectively, and grew for 24 hrs on SC-Ura medium containing 25 mM NaHCO 3 with pH value as indicated. pH 5.8 was the control … Web1 aug. 2024 · Evaluation of tolerance of INVSc1 to drought, salt, and H 2 O 2 stress. The positive colony was diluted with SDG solution and cultivated at 30 °C for about 36–40 h … flocked nasopharyngeal swab testing procedure

质粒表达载体pCMV-BD载体 pCMV-AD载体 pBD-p53载体 pAD …

Category:pYES2/CT, pYES3/CT, and pYC2/CT - iGEM

Tags:Invsc1 yeast strain

Invsc1 yeast strain

Commonly used strains - SGD-Wiki

Web6 mei 2024 · The pYES2– MeGLYI-13 vector was then transferred into the INVSc1 yeast strain via the PEG/LiAc transformation method. To investigate the role of MeGLYI-13 in … WebINVSC1 Yeast Strains Alternative name INVSC1 Yeast Strains,INVSC1,Yeast Packing 100ul Storage at -20DEG C Strains Resistance Kan Culture Medium YPDA Condition …

Invsc1 yeast strain

Did you know?

WebYeast cells (InvSc1) were transformed with 500 ng of the 59leu2TTRP1T39leu2 frag-ment and 25 ng of linearized pDEST32 with LEU marker ... rescued into the PJ69-4 MATalpha haploid yeast strain. The Piromyces XI gene was cloned into the resulting TRP select-able modified bait plasmid using standard Gateway recombi-

WebThird, the Yersinia pestis xylulokinase gene was cloned into the LEU-selectable SUMO vector and placed into the INVSc1-XI-Lyt-1 yeast. Yeast strains expressing XI and … Web27 feb. 2016 · The yeast INVScI bacterium grew significantly better than the control strain under NaCl, NaHCO 3, and H 2 O 2 treatments. These findings illustrated that OsLOL5 overexpression enhanced yeast resistance for SA stress through active oxygen species.

Web12 mrt. 2007 · The INVSc1 strain is a fast-growing diploid strain ideal for expression. It has the following genotype: MATá his3D1 leu2 trp1-289 ura3-52 MAT his3D1 leu2 trp1-289 ura3-52. Yeast INVSc1 is not appropriate … Web15 jun. 2024 · The S. cerevisiae INVSc1 strain with empty plasmids was set as a control strain. The successfully constructed S. cerevisiae INVSc1 transformants were precultured in SC-U medium with 2% (w/v) glucose at 30 °C, and transferred into SC-U induction medium for overexpression of the target genes.

WebThe recombinant expression plasmids were transferred into INVSc1 and PCC7942 respectively, and the transgenic strains were screened. The similar number of transgenic yeast and transgenic cyanobacteria cells were cultured with addition of the same volume of linoleic acid (LA).

WebInvitrogen™ INV Sc 1, S. cerevisiae Yeast Strain Schnell wachsender diploider Stamm, der sich hervorragend für die Expression eignet Marke: Invitrogen™ C81000 Weitere Details … flocked noble christmas treeWebSaccharomyces cerevisiae yeast cells serve as a model to elucidate the bases of salt tolerance and potassium homeostasis regulation in eukaryotic cells. In this study, we show that two widely used laboratory strains, BY4741 and W303-1A, differ not only in cell size and volume but also in their relat … great lakes shark sock coWeb18 dec. 2012 · We used several yeast strains for terpene synthase expression; INVSc1 (Invitrogen), EPY219 and EPY224, which are from the Keasling lab . EPY219 and … great lakes sheep show and saleWeb5 jan. 2024 · The reconstructed plasmids and empty plasmid were then transformed into yeast strain INVSc1 using lithium acetate-polyethylene glycol-mediated transformation procedure. The positive transgenic clones were screened and cultivated in yeast synthetic drop-out medium (SD/-Ura) with 2% ( w/v ) glucose at 30 °C for 12 h. great lakes shawarma company near meWebINV Sc 1, S. cerevisiae Yeast Strain Catalog number: C81000 Related applications: Protein Expression Technical Support Customer Service Save to list Add to cart Product … great lakes ship artWeb我们提供INVSc1酵母株。这是一种二倍体酵母株，仅作表达用途。该酵母株不能形成良好的孢子，因此不适用于酵母遗传研究。INVSc1酵母株的基因型和表型如下： 基因 … great lakes shark sock companyWebItem INVSc1, S. cerevisiae Yeast Strain; Company Thermo Fisher Scientific; Catalog Number C81000; This product is no longer available on Biocompare. Biocompare is the … flocked ornaments